Stimulation of macrophage function by interphotoreceptor retinoid-binding protein: production of nitric oxide
Korean Journal of Ophthalmology
;
: 57-64, 1999.
Artigo
em Inglês
| WPRIM
| ID: wpr-119578
ABSTRACT
In this study, we investigated whether retinal soluble proteins, such as interphotoreceptor retinoid-binding protein(IRBP), play a role in the induction of nitric oxide by macrophages in vitro. Cells from the murine macrophage cell line RAW 264.7 and rat and rabbit peritoneal macrophages were incubated in the presence of retinal soluble protein. The nitrite level in the cultured supernatant was evaluated for nitric oxide production using the Griess reaction. IRBP induced significant, dose-dependent nitrite production in both RAW 264.7 and rat peritoneal macrophages. Induction of inducible nitric oxide synthase (iNOS) by retinal proteins was inhibited by the iNOS-specific inhibitor, aminoguanidine, and the tyrosine inhibitor, genistein. These results show that soluble retinal proteins significantly induce nitric acid production by macrophages. Increased production of reactive oxygen species by macrophages in the presence of this soluble retinal protein in vivo may accelerate photoreceptor degeneration in uveitis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ratos Endogâmicos Lew
/
Proteínas de Ligação ao Retinol
/
Estudo Comparativo
/
Linhagem Celular
/
Macrófagos Peritoneais
/
Óxido Nítrico Sintase
/
Inibidores Enzimáticos
/
Proteínas do Olho
/
Guanidinas
/
Animais
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Journal of Ophthalmology
Ano de publicação:
1999
Tipo de documento:
Artigo
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