Detection of RTP801, a Gene That is Differentially Expressed in Irradiated HeLa Cells / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 263-270, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-119629
ABSTRACT
PURPOSE:
To quantify the effects of irradiation on the expression levels of a differentially expressed gene, RTP801, in HeLa cells. MATERIALS ANDMETHODS:
Total RNA was isolated from irradiated and non-irradiated HeLa cells. A subtraction library was constructed, from which 88 random clones were screened. The expression patterns of one clone, detected by reverse Northern blotting, were quantified by real time RT-PCR, using CYBR green I dye.RESULTS:
RTP801, a hypoxia-inducible factor-I-responsive gene, was identified as a differentially expressed gene in HeLa cells exposed to X-ray. Real time RT-PCR showed that the mRNA levels of RTP801 were greatly diminished by radiation.CONCLUSIONS:
These results suggest that down-regulation of hypoxia-inducible factor-I-responsive genes, such as RTP801, in irradiated HeLa cells may result in reductions in the radiotherapy resistance of tumor cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Radioterapia
/
RNA
/
RNA Mensageiro
/
Células HeLa
/
Regulação para Baixo
/
Northern Blotting
/
Genes vif
/
Células Clonais
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Cancer Research and Treatment
Ano de publicação:
2004
Tipo de documento:
Artigo
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