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The role of Rho GTPases in the regulation of the rearrangement of actin cytoskeleton and cell movement
Experimental & Molecular Medicine ; : 358-366, 2004.
Artigo em Inglês | WPRIM | ID: wpr-119640
ABSTRACT
The rearrangement of the actin cytoskeleton has been shown to play a critical role in the development of transformation and malignant phenotype of cancer cells. Rho family GTPases regulate the arrangement of the actin cytoskeleton. By wound-healing assay, we have found that NIH 3T3 fibroblast cells move towards the wound- gaps by extending filopodial and lamellipdial structures at the leading edge of the moving cells. We have inactivated the function of Rho GTPases of v-Ras transformed NIH 3T3 cells by overexpressing Rho GTPase-activating (RhoGAP) domain of RhoGAP of p190. We have observed that inactivation of Rho, Rac and Cdc42 GTPases by overexpressing RHG causes inhibition of (i) polymerization of actin to form filaments, (ii) formation of lamellipodia, filopodia and stress fibres, (iii) cell motility, (iv) cell spreading and (v) cell-to-cell adhesions. These results further strengthen the current knowledge on the role of Rho, Rac and Cdc42 GTPases in the regulation of the rearrangement of actin cytoskeleton. Our results, for the first time, demonstrate that RhoGAP domain of RhoGAP could be used to study the molecular mechanism of Ras-mediated signalling in growth, differentiation and carcinogenesis.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Cicatrização / Bioensaio / Citoesqueleto de Actina / Linhagem Celular Transformada / Movimento Celular / Transformação Celular Neoplásica / Proteínas rho de Ligação ao GTP / Células NIH 3T3 Limite: Animais Idioma: Inglês Revista: Experimental & Molecular Medicine Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Cicatrização / Bioensaio / Citoesqueleto de Actina / Linhagem Celular Transformada / Movimento Celular / Transformação Celular Neoplásica / Proteínas rho de Ligação ao GTP / Células NIH 3T3 Limite: Animais Idioma: Inglês Revista: Experimental & Molecular Medicine Ano de publicação: 2004 Tipo de documento: Artigo