Altered Regulation of Aquaporin-2 Water Channels in the Kidney Following Acute Blood Volume Depletion in Rats / 대한신장학회잡지
Korean Journal of Nephrology
;
: 37-42, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-12020
ABSTRACT
BACKGROUND:
The present study was aimed to examine the regulation of aquaporin (AQP)-2 water channels in the kidney following blood volume depletion.METHODS:
Male Sprague-Dawley rats were acutely blood volume-depleted by withdrawal of arterial blood up to 2% of body weight. The expression of AQP2 mRNA and protein was determined by reverse transcription - polymerase chain reaction and Western blot analysis, respectively, in the inner medulla of the kidney 1 and 3 hours after the hemorrhage.RESULTS:
The mRNA expression of AQP2 was significantly increased 1 hour after the bleeding. However, neither the shuttling nor the total abundance of AQP2 proteins was significantly altered. On the contrary, 3 hours after the bleeding, the expression of AQP2 proteins as well as that of AQP2 mRNA was significantly increased. The shuttling of AQP2 proteins was also increased.CONCLUSION:
These results suggest that an increased expression of AQP2 channels in the kidney may confer one of compensatory mechanisms restoring the circulating volume in an acute hypovolemic state.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Volume Sanguíneo
/
Peso Corporal
/
RNA Mensageiro
/
Western Blotting
/
Reação em Cadeia da Polimerase
/
Ratos Sprague-Dawley
/
Aquaporinas
/
Hipovolemia
/
Transcrição Reversa
/
Aquaporina 2
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Korean Journal of Nephrology
Ano de publicação:
2003
Tipo de documento:
Artigo
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