Expression and localization of Rdd proteins in Xenopus embryo / 대한해부학회지
Anatomy & Cell Biology
;
: 18-27, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-121388
ABSTRACT
The previous study has shown that repeated D domain-like (Rdd) proteins, a group of novel secretory proteins consisting of repeated domains of a cysteine-rich sequence, are involved in the process of blood vessel formation in Xenopus embryo. We performed further experiments to examine the localization of Rdd proteins in embryogenesis. Detection of tagged Rdd proteins expressed in blastomeres showed that Rdd proteins formed a high molecular weight complex and existed in the extracellular space. A rabbit antibody against the Rdd synthetic peptide identified a single band of 28 kD in embryonic tissue extract. By whole-mount immunostaining analysis, signal was detected in the regions of inter-somites, vitelline veins, and branchial arches at the tailbud stage. Staining of Rdd was remarkably reduced in the embryos injected with vascular endothelial growth factor Morpholino. We suggest that Rdd proteins interact with a molecule(s) associated with vascular precursor cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Veias
/
Xenopus
/
Blastômeros
/
Vasos Sanguíneos
/
Região Branquial
/
Fator A de Crescimento do Endotélio Vascular
/
Vitelinas
/
Desenvolvimento Embrionário
/
Estruturas Embrionárias
/
Espaço Extracelular
Tipo de estudo:
Estudo prognóstico
Limite:
Gravidez
Idioma:
Inglês
Revista:
Anatomy & Cell Biology
Ano de publicação:
2014
Tipo de documento:
Artigo
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