Expression of Fibronectin, Vitronectin, Surfactant-A and D in Interaction of Pneumocystis carinii and Alveolar Epithelial Cells in Pneumocystis carinii Pneumonia
Korean Journal of Pathology
;
: 625-635, 2000.
Artigo
em Coreano
| WPRIM
| ID: wpr-121439
ABSTRACT
Both fibronectin and vitronectin bind to Pneumocystis carinii (P. carinii) and mediate the attachment of the organisms to respiratory epithelial cells. Surfactant A and D play a role in the interaction between P. carinii and host cells. In this study we examined the expression of fibronectin, vitronectin, surfactant-A and D in the interaction between P. carinii and alveolar epithelial cells by immunohistochemistry and pre-embedding immunoelectron microscopy. The experimental rat model of P. carinii pneumonia was induced by administration of low protein diet (8%) and drinking water containing dexamethasone (2 mg/liter) for 6 to 8 weeks. The primary antibodies for light and electron microscopic immunohistochemistries were monoclonal antibodies including fibronectin (1100) and vitronectin (1100), and polyclonal antibodies including surfactant A (150) and D (150), respectively. Light microscopic immunohistochemistry for the fibronectin, vitronectin, surfactant-A and D showed strong expressions on the P. carinii and surface linings of type I alveolar epithelial cells. The electron microscopic immunohistochemistry of the fibronectin and vitronectin showed a strong immunoexpression along the surface pellicles and tubular extensions of P. carinii trophozoites, and surface membranes of the type I epithelial cells. The surfactant-A and D proteins showed a strong expression on the pellicles of P. carinii and surface membranes of the type I epithelial cells, but a weak expression on the free-floating surfactant materials. In conclusions, the trophozoites of P. carinii were mostly attached to type I epithelial cells. The fibronectin, vitronectin, surfactant-A and D were strongly expressed, and played an enhancing role in the binding between the P. carinii organisms and the type I alveolar epithelial cells.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pneumocystis
/
Pneumonia
/
Pneumonia por Pneumocystis
/
Água Potável
/
Dexametasona
/
Imuno-Histoquímica
/
Microscopia Imunoeletrônica
/
Fibronectinas
/
Dieta com Restrição de Proteínas
/
Vitronectina
Idioma:
Coreano
Revista:
Korean Journal of Pathology
Ano de publicação:
2000
Tipo de documento:
Artigo
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