Immunogenecity of Outer Membrane Proteins of vibro vulnificus
Journal of the Korean Society for Microbiology
;
: 353-360, 1998.
Artigo
em Coreano
| WPRIM
| ID: wpr-121529
ABSTRACT
To provide basic information on the pathogengenesis of Vibrio vulnificus infection and for the manufacture of effective vaccine, outer membrane proteins (OMPs) were extracted from V. vulnificus ATCC 27562 strain and analysed by 12% of sodium dodecyl sulfate-polyarylamide gel(SDS-PAGE). Major 36 kDa and 48 kDa, 46 kDa, 66 kDa and 24 kDa protein bands appeared on gel by Coomassie stain and determined by densitometer. Immunogenecity of these proteins was examined by enzyme-linked immunosorbant assay(ELISA) and western blotting with rabbit anti-V. vulnificus serum. OMPs reacted with this antiserum, and the major 36 kDa protein appeared most immunogenic.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sódio
/
Ensaio de Imunoadsorção Enzimática
/
Western Blotting
/
Vibrio vulnificus
/
Proteínas de Membrana
/
Membranas
Idioma:
Coreano
Revista:
Journal of the Korean Society for Microbiology
Ano de publicação:
1998
Tipo de documento:
Artigo
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