Analysis of Five Coding Region Polymorphism in Mitochondrial DNA / 대한법의학회지
Korean Journal of Legal Medicine
;
: 56-61, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-122672
ABSTRACT
For evaluation of the five coding region (CR) polymorphism in mitochondrial DNA (mtDNA); we had performed PCR and direct sequencing in 599 unrelated Korean who showed the identical DNA type in D-loop mitochondrial DNA analysis for total 2,810 bp fragment. Following the sequence analysis, all the sequences of five regions were compared respectively to Anderson standard sequence to investigate the nucleotide variations. The result showed, a total 4,565 nucleotide variations were observed at 190 positions in five CR as 3,931 (86.11%) substitutions, 32 (0.7%) insertions, and 602 (13.19%) deletions and the allele diversities (h) were higher than 0.9992 when adding each CR or combined CR to D-loop analysis in mtDNA. In conclusion, we could confirm the five CR are useful for forensic testing through the nucleotide variation and hapolotypes polymorphism.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
DNA Mitocondrial
/
Reação em Cadeia da Polimerase
/
Análise de Sequência
/
Alelos
/
Codificação Clínica
Idioma:
Coreano
Revista:
Korean Journal of Legal Medicine
Ano de publicação:
2003
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS