Comparison of Proteome Component of Helicobacter pylori in Different Atmospheric CO2 Concentration
Journal of Bacteriology and Virology
;
: 213-224, 2007.
Artigo
em Coreano
| WPRIM
| ID: wpr-123859
ABSTRACT
Helicobacter pylori is a spiral, slow growing gram-negative microaerophilic bacterium. It has been shown to be the etiological agent of gastroduodenal diseases, such as chronic gastritis, gastric and duodenal ulcers, and gastric cancer. General culture condition of H. pylori is 5% O2, 10% CO2 and 100% humid atmosphere. We have compared proliferation protein expression profile of H. pylori incubated under normal microaerophilic (10% CO2) and environment stress (4% CO2, 18% CO2) conditions. H. pylori cultured under environment stress displayed coccoid morphology and timedependent decrease in proliferation. We have further compared the protein expression profiles of H. pylori under normal growing and environment stress conditions by a global proteomic analysis, which includes high-resolution 2-DE followed by matrix-assisted laser desorption/ionization time of flight and nanoelectrospray/tandem mass spectrometry. In total, 42 protein spots were found to be up- or down-regulated by more than 2-fold under environment stress conditions. Of the 42 protein spots processed, 27 spots were identified; they represented 19 genes, including 2 kinds of hypothetical proteins.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Atmosfera
/
Espectrometria de Massas
/
Neoplasias Gástricas
/
Helicobacter pylori
/
Helicobacter
/
Proteoma
/
Úlcera Duodenal
/
Gastrite
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2007
Tipo de documento:
Artigo
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