A known expressed sequence tag, BM742401, is a potent lincRNA inhibiting cancer metastasis
Experimental & Molecular Medicine
;
: e31-2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-124615
ABSTRACT
Long intergenic non-coding RNAs (lincRNAs) have historically been ignored in cancer biology. However, thousands of lincRNAs have been identified in mammals using recently developed genomic tools, including microarray and high-throughput RNA sequencing (RNA-seq). Several of the lincRNAs identified have been well characterized for their functions in carcinogenesis. Here we performed RNA-seq experiments comparing gastric cancer with normal tissues to find differentially expressed transcripts in intergenic regions. By analyzing our own RNA-seq and public microarray data, we identified 31 transcripts, including a known expressed sequence tag, BM742401. BM742401 was downregulated in cancer, and its downregulation was associated with poor survival in gastric cancer patients. Ectopic overexpression of BM742401 inhibited metastasis-related phenotypes and decreased the concentration of extracellular MMP9. These results suggest that BM742401 is a potential lincRNA marker and therapeutic target.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Neoplasias Gástricas
/
RNA Mensageiro
/
Regulação Neoplásica da Expressão Gênica
/
Modelos de Riscos Proporcionais
/
Análise de Sobrevida
/
Análise Multivariada
/
Reprodutibilidade dos Testes
/
Predisposição Genética para Doença
/
Etiquetas de Sequências Expressas
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2013
Tipo de documento:
Artigo
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