Protein Kinase C-delta Mediates Nitric Oxide- induced Proliferation of Gastric Cancer Cells, MKN-2
Journal of the Korean Surgical Society
;
: 194-200, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-125361
ABSTRACT
PURPOSE:
The purpose of this study was to investigate the effect of exogenous nitric oxide (NO) on the proliferation of gastric carcinoma cells and the signaling pathways that regulate these responses.METHODS:
MKN-28 cells were obtained from the Korean Cell Line Bank (KCLB) and maintained in DMEM culture media. The effect of sodium nitroprusside (SNP), a NO donor, on the proliferation of a serum-starved gastric carcinoma cell line, MKN-28, was examined by [3H]thymidine incorporation. Western blot was performed to analyze the translocation of protein kinase C (PKC)-deltafrom the cytosol to the plasma membrane of the MKN-28 cells.RESULTS:
The proliferation of MKN-28 cells was significantly increased by SNP. It was also found that the proliferation was significantly inhibited by the protein kinase A (PKA) inhibitor, KT5720, and the protein kinase G inhibitor (PKG), KT5823, in SNP-treated cells. The SNP-induced proliferation was also inhibited by the PKC-deltaspecific inhibitor, rottlerin (1mu), but was increased by the PKC-beta inhibitor, Go6976 (1muM). The amount of translocated PKC-deltaprotein in the plasma membrane from the cytosol increased time-dependently after treating the cells with SNP, suggesting that NO activates PKC-delta Anti-inflammatory drugs, including dexamethasone, aspirin, indomethacin, mephenamic acid, and acetaminophen inhibited the SNP-induced proliferation of the cells and blocked of PKC-deltaactivation.CONCLUSION:
NO stimulates the proliferation of serum- starved gastric cancer cells. The NO-induced proliferation may be mediated by PKC-delta The inhibitory effect of anti-inflammatory drugs on cell proliferation may be related to the inhibition of PKC-deltaactivity.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Quinases
/
Neoplasias Gástricas
/
Doadores de Tecidos
/
Proteína Quinase C
/
Dexametasona
/
Nitroprussiato
/
Linhagem Celular
/
Membrana Celular
/
Aspirina
/
Indometacina
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Surgical Society
Ano de publicação:
2003
Tipo de documento:
Artigo
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