Your browser doesn't support javascript.
loading
Performance of the BacT Alert 3D System Versus Solid Media for Recovery and Drug Susceptibility Testing of Mycobacterium tuberculosis in a Tertiary Hospital in Korea / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases ; : 282-288, 2016.
Artigo em Inglês | WPRIM | ID: wpr-125740
ABSTRACT

BACKGROUND:

Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing.

METHODS:

Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on Löwenstein-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system.

RESULTS:

The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%).

CONCLUSION:

The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Rifampina / Escarro / Tuberculose / Resistência a Medicamentos / Resistência Microbiana a Medicamentos / Diagnóstico / Tuberculose Extensivamente Resistente a Medicamentos / Centros de Atenção Terciária / Isoniazida / Coreia (Geográfico) Tipo de estudo: Estudo diagnóstico Limite: Humanos País/Região como assunto: Ásia Idioma: Inglês Revista: Tuberculosis and Respiratory Diseases Ano de publicação: 2016 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Rifampina / Escarro / Tuberculose / Resistência a Medicamentos / Resistência Microbiana a Medicamentos / Diagnóstico / Tuberculose Extensivamente Resistente a Medicamentos / Centros de Atenção Terciária / Isoniazida / Coreia (Geográfico) Tipo de estudo: Estudo diagnóstico Limite: Humanos País/Região como assunto: Ásia Idioma: Inglês Revista: Tuberculosis and Respiratory Diseases Ano de publicação: 2016 Tipo de documento: Artigo