The Effect of Tamoxifen of the Estrogen Receptor cDNA-Iipofected MDA-MB-231 Human Breast Cancer Cells / 한국유방암학회지
Journal of Korean Breast Cancer Society
;
: 192-202, 1998.
Artigo
em Coreano
| WPRIM
| ID: wpr-126315
ABSTRACT
BACKGROUND:
The loss of estrogen and progesterone receptors appears to be associated with a progression to less-differentiated and hormone-independent tumors. The gain of hormone independency over time even in estrogen receptor-positive tumors has become another obstacle to endocrine therapy for breast cancer. We tried to regain the hormone dependency in estrogen receptor-negative breast cancer cells by lipofecting estrogen receptor cDNA. MATERIALS ANDMETHODS:
The mutant human estrogen receptor cDNA (pSG5-HE0) was lipofected into estrogen receptor-negative human breast cancer cell line MDA-MB-231, in an attempt to restore their sensitivity to antiestrogen. Then the effects if 17 beta -estradiol and tamoxifen were studied by counting viable cell numbers after treating the lipofected cell line with either one or together.RESULTS:
The cell growth was most profoundly inhibited in 4 days after lipofection with mutant human estrogen receptor cDNA, which was overcome after that days. Tamoxifen, as an antiestrogen, showed a growth inhibitory effect slightly strong over combined conditions of tamoxifen and 17 beta estradiol compared to estrogen-treated group and to control.CONCLUSIONS:
The temporary induction of estrogen receptor by lipofection with pSG5-HE0 on estrogen receptor-negative human breast cancer cell line MDA-MB-231 showed negative growth control on these cells by tamoxifen, indicating that liposome-mediated estrogen receptor transfection may be used as a novel therapeutic strategy for hormone independent human breast cancers in the near future.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tamoxifeno
/
Mama
/
Neoplasias da Mama
/
Transfecção
/
Terapia Genética
/
Receptores de Progesterona
/
Contagem de Células
/
Linhagem Celular
/
DNA Complementar
/
Moduladores de Receptor Estrogênico
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of Korean Breast Cancer Society
Ano de publicação:
1998
Tipo de documento:
Artigo
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