Molecular Phylogenetics of Exophiala Species Isolated from Korea
Annals of Dermatology
;
: 287-294, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-126695
ABSTRACT
BACKGROUND:
Recently, identification of fungi have been supplemented by molecular tools, such as ribosomal internal transcribed spacer (ITS) sequence analysis. According to these tools, morphological Exophiala species was newly introduced or redefined.OBJECTIVE:
This study was designed to investigate the phylogenetics based on ribosomal ITS sequence analysis from clinical Exophiala species isolated in Korea.METHODS:
The strains of Exophiala species were 4 clinical isolates of phaeohyphomycosis agents kept in the department of dermatology, Dongguk University Medical Center(DUMC), Gyeongju, Korea. The DNAs of total 5 strains of Exophiala species were extracted by bead-beating method. Polymerase chain reaction of ITS region using the primer pairs ITS1-ITS4, was done and phylogenetic tree contributed from sequences of ITS region from 5 Korean isolates including E. dermatitidis CBS 109154 and comparative related strains deposited in GenBank.RESULTS:
The strains of Exophiala species were 3 strains of E. dermatitidis, 1 strain of E. jeanselmei and 1 strain of Exophiala new species. Among the 3 subtypes (type A, B, C) of E. jeanselmei, E. jeanselmei DUMC 9901 belonged to type B. Of the 2 main types of E. dermatitidis (type A, B) and 3 subtypes of E. dermatitidis type A (A0, A1 and A2), two strains (E. dermatitidis CBS 709.95, E. dermatitidis CBS 109154) belonged to A0 subtypes, 1 strain (E. dermatitidis DUMC 9902) A1 subtype, respectively.CONCLUSION:
Phylogenetic analysis of ITS region sequence provided useful information not only for new species identification but for the subtyping and origin of Exophiala species.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Entorses e Distensões
/
Exophiala
/
DNA
/
Reação em Cadeia da Polimerase
/
Análise de Sequência
/
Dermatologia
/
Feoifomicose
/
Fungos
/
Coreia (Geográfico)
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Annals of Dermatology
Ano de publicação:
2012
Tipo de documento:
Artigo
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