Surveillance of Stool Samples for the Presence of Enterobacter sakazakii among Korean People
Yonsei Medical Journal
;
: 1017-1022, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-126735
ABSTRACT
PURPOSE:
Enterobacter sakazakii (E. sakazakii) infections are an important cause of life-threatening meningitis, septicemia, and necrotizing enterocolitis in infants. Dried infant formula milk is an important vehicle for E. sakazakii infection. E. sakazakii was isolated in Korea from dried infant formula milk. Although E. sakazakii infection of infants may occur in Korea, its prevalence has not yet been documented. Therefore, we determined the prevalence of E. sakazakii and documented symptoms. MATERIALS ANDMETHODS:
Between March and October 2006, 1,146 stool samples were collected from patients at Uijeongbu St. Mary's Hospital. Each fecal swab was dissolved in 10mL of buffered peptone solution, and enriched culture was streaked onto Druggan-Forsythe-Iversen (DFI) agar. Presumptive E. sakazakii colonies that exhibited a blue-green color during culture on DFI medium were selected. The identity of colonies that developed yellow pigment during culture on TSA was determined using the Vitek system and PCR.RESULTS:
We isolated 4 E. sakazakii strains whose 16S rRNA sequence alignments had a similarity of 99% with those of 3 E. sakazakii ATCC strains.CONCLUSION:
This is the first report on isolation of E. sakazakii from stool samples and to document the symptoms of Korean patients.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Bacteriano
/
Dados de Sequência Molecular
/
RNA Ribossômico 16S
/
Sequência de Bases
/
Homologia de Sequência do Ácido Nucleico
/
Farmacorresistência Bacteriana Múltipla
/
Cronobacter sakazakii
/
Infecções por Enterobacteriaceae
/
Fezes
/
Coreia (Geográfico)
Tipo de estudo:
Estudo de rastreamento
Limite:
Adolescente
/
Adulto
/
Idoso
/
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Lactente
/
Masculino
/
Recém-Nascido
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2008
Tipo de documento:
Artigo
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