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Effects of 4 Weeks Recombinant Human Growth Hormone Administration on Insulin Resistance of Skeletal Muscle in Rats
Yonsei Medical Journal ; : 1008-1016, 2008.
Artigo em Inglês | WPRIM | ID: wpr-126736
ABSTRACT

PURPOSE:

Effect of recombinant human growth hormone (rhGH) administration on lipid storage, and its subsequent effect on insulin sensitivity have not yet been adequately examined. Thus, we investigated the effects of rhGH treatment on muscle triglyceride (TG) and ceramide content, and insulin sensitivity after 4 weeks of rhGH administration in rats. MATERIALS AND

METHODS:

Fourteen rats were randomly assigned to two groups rhGH injection group (GH, n = 7) and saline injection group (CON, n = 7). GH received rhGH by subcutaneous injections (130microgram/kg(-1)/day(-1), 6 days/week(-1)) for 4 weeks, while CON received saline injections that were equivalent in volume to GH group. Intramuscular TG and ceramide content and hepatic TG content were measured. To determine insulin sesitivity, oral glucose tolerance test (OGTT) and muscle incubation for glucose transport rate were performed in rats, and used as indicators of insulin sensitivity. We also examined plasm lipid profiles.

RESULTS:

After 4 weeks of rhGH treatment, the GH group had higher muscle and liver TG contents than the CON (p < 0.05). Ceramide content in GH was significantly greater than that in CON (p < 0.05). GH also had higher plasma levels of FFA (p < 0.05), glucose and insulin responses during OGTT (p < 0.05), and lower glucose transport rates in submaximal insulin concentration (p < 0.05) as compared with CON. Results indicate that rhGH treatment is associated with insulin resistance in rats.

CONCLUSION:

rhGH treatment elevated muscle TG and ceramide content, and hepatic TG content. Thus, elevation of these compounde by rhGH treatment could contribute to the development of insulin resistance in rats.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Triglicerídeos / Proteínas Recombinantes / Resistência à Insulina / Ceramidas / Ratos Sprague-Dawley / Músculo Esquelético / Hormônio do Crescimento Humano / Transportador de Glucose Tipo 4 / Glucose Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Yonsei Medical Journal Ano de publicação: 2008 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Triglicerídeos / Proteínas Recombinantes / Resistência à Insulina / Ceramidas / Ratos Sprague-Dawley / Músculo Esquelético / Hormônio do Crescimento Humano / Transportador de Glucose Tipo 4 / Glucose Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Yonsei Medical Journal Ano de publicação: 2008 Tipo de documento: Artigo