Proteomic Analysis of DJ-1 Expression in the Muscles of Spastic Cerebral Palsy Patients / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 45-55, 2008.
Artigo
em Coreano
| WPRIM
| ID: wpr-126983
ABSTRACT
PURPOSE:
Using proteomic analysis, this study was performed to see the characteristics of proteins expression in the muscles of spastic cerebral palsy patients. MATERIALS ANDMETHODS:
We studied twelve specimens from six patients with spastic cerebral palsy, three patients with myelomeningocele, and three normal people who underwent orthopaedic surgeries due to trauma. We studied the extracted proteins showing differences in the two-dimensional electrophoresis, and the prominent thirteen proteins were re-evaluated by proteomics and the reverse transcriptional polymerase chain reaction, which was to clarify the relationship between gene and protein expression.RESULTS:
Among fifteen proteins, six proteins were found to be higher in normal people, and nine were found to be higher in the groups of patients by spot histogram. The results of proteomic analysis with MALDI-TOF for fifteen proteins showed that the expression of DJ-1 was related to cerebral palsy.CONCLUSION:
This study shows that strong expression of DJ-1 is related to spasticity and cerebral palsy. We showed for the first time the possibility of any relationship between spastic condition and DJ-1 expression.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas
/
Paralisia Cerebral
/
Reação em Cadeia da Polimerase
/
Meningomielocele
/
Proteômica
/
Eletroforese
/
Espasticidade Muscular
/
Músculos
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of Korean Orthopaedic Research Society
Ano de publicação:
2008
Tipo de documento:
Artigo
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