p38 Mitogen-Activated Protein Kinase and Extracellular Signal-Regulated Kinase Regulate Nitric Oxide Production and Inflammatory Cytokine Expression in Raw Cells
Immune Network
;
: 30-35, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-127002
ABSTRACT
BACKGROUND:
p38 and extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK) signaling are thought to have critical role in lipopolysaccharide (LPS)-induced immune response but the molecular mechanism underlying the induction of these signaling are not clear.METHODS:
Specific inhibitors for p38, SB203580, and for ERK, PD98059 were used. Cells were stimulated by LPS with or without specific MAPK inhibitors.RESULTS:
LPS activated inducible nitric oxide synthase (iNOS), subsequent NO productions, and pro-inflammatory cytokine gene expressions (TNF-alpha, IL-1beta, IL-6, and IL-12). Treatment of both SB203580 and PD98059 decreased LPS-induced NO productions. Concomitant decreases in the expression of iNOS mRNA and protein were detected. SB203580 and PD98059 decreased LPS-induced gene expression of IL-1beta and IL-6. SB203580 increased LPS-induced expression of TNF-alpha and IL-12, and reactive oxygen species production, but PD98059 had no effect.CONCLUSION:
These results indicate that both p38 and ERK pathways are involved in LPS-stimulated NO synthesis, and expression of IL-1beta and IL-6. p38 signaling pathways are involved in LPS-induced TNF-alpha and IL-12, and reactive oxygen species plays an important role in these signaling in macrophage.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfotransferases
/
Proteínas Quinases
/
RNA Mensageiro
/
Expressão Gênica
/
Interleucina-6
/
Fator de Necrose Tumoral alfa
/
Espécies Reativas de Oxigênio
/
Interleucina-12
/
Sistema de Sinalização das MAP Quinases
/
Óxido Nítrico Sintase Tipo II
Idioma:
Inglês
Revista:
Immune Network
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS