Immunologic reactivity of a lipopolysaccharide-protein complex of type A Pasteurella multocida in mice
Journal of Veterinary Science
;
: 87-95, 2000.
Artigo
em Inglês
| WPRIM
| ID: wpr-128400
ABSTRACT
The immunologic reactivity of a lipopolysaccharide (LPS)-protein complex isolated from a potassium thiocyanate extract of a Pasteurella multocida (capsular type A and somatic type 3) strain was evaluated in mice. The LPS-protein complex provided 100% protection in mice against a challenge with the homologous strain. However, when the complex was fractionated into LPS and protein moieties by phenol-water treatment, both components lacked immunogenicity. The complex and extracted components were mitogenic for mouse B lymphocytes with the protein moiety the most active. Although immune serum against the LPS-protein complex protected mice against challenge thereby indicating a role for humoral immunity, the LPS-protein complex of P. multocida was also found to induce cell-mediated immunity. This cell-mediated immunity was demonstrated in mice immunized with the complex by (1). mitogenic responses of T lymphocytes, (2). induction of delayed type hypersensitivity reaction in the hind footpads, and (3). enhanced resistance to challenge infection with Salmonella enteritidis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Infecções por Pasteurella
/
Salmonella enteritidis
/
Salmonelose Animal
/
Baço
/
Proteínas de Bactérias
/
Ativação Linfocitária
/
Lipopolissacarídeos
/
Imunização Passiva
/
Pasteurella multocida
/
Fracionamento Químico
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2000
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS