The Effect of Ischemic Preconditioning in Rat Liver: The Expression of Interleukin-1 and Nuclear Factor-B
Korean Journal of Pathology
;
: 238-242, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-128464
ABSTRACT
BACKGROUND:
A short period of ischemia and reperfusion, called ischemic preconditioning, protects various tissues against subsequent sustained ischemic insult. Apoptosis of hepatocytes and sinusoidal endothelial cells are a critical mechanisms of injury in the ischemic liver. Because nuclear factor-B (NF-B) has a significant role in the cell survival, we hypothesized that ischemic preconditioning protects by inhibition of apoptosis through the expression of NF-B, induced by interleukin-1 (IL-1), which is known for enhancement of its transcription and activation.METHODS:
We induced ischemia and reperfusion on rat liver, and performed in situ terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labelling assay and polymerase chain reaction for IL-1 mRNA and NF-B mRNA.RESULTS:
Apoptosis of hepatocytes and sinusoidal endothelial cells, assessed by in situ TUNEL assay, was significantly reduced with preconditioning. The expression of IL-1 mRNA and NF-B mRNA are seen on discrete monoclonal bands around 344 and 356 base pairs, in comparison with normal rat liver, but, there was no significant difference between the ischemia-reperfusion group and the preconditioning group.CONCLUSIONS:
We suggest that ischemic preconditioning confers dramatic protection against prolonged ischemia via inhibition of apotosis through the expression of IL-1 inducing NF-B and its activation. However, we need further study in the activity of NF-B, such as nucleotide shift assay, because the activity of NF-B is regulated by binding of the inhibitory protein, IB.
Texto completo:
DisponíveL
Índice:
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Assunto principal:
RNA Mensageiro
/
Reperfusão
/
Sobrevivência Celular
/
Reação em Cadeia da Polimerase
/
Interleucina-1
/
Apoptose
/
Precondicionamento Isquêmico
/
Marcação In Situ das Extremidades Cortadas
/
Pareamento de Bases
/
Hepatócitos
Limite:
Animais
Idioma:
Coreano
Revista:
Korean Journal of Pathology
Ano de publicação:
2002
Tipo de documento:
Artigo
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