Nitric Oxide-Induced Intracellular Ca2+ Modulation in Macrovascular Endothelial Cells
Korean Circulation Journal
;
: 600-609, 2004.
Artigo
em Coreano
| WPRIM
| ID: wpr-128653
ABSTRACT
BACKGROUND AND OBJECTIVES:
Nitric oxide (NO) reduces the intracellular Ca2+ concentration ([Ca2+]i) in smooth muscle cells, whereas the effect of NO on [Ca2+]i in endothelial cells is still controversial. Therefore, the effect of NO on the [Ca2+]i, and its mechanism in mouse aortic endothelial cells (MAEC) and human umbilical vein endothelial cells (HUVEC) were examined. MATERIALS ANDMETHODS:
In primary cultured MAEC and HUVEC, cells were loaded with fura 2-AM and [Ca2+]i and measured using a microfluorometer.RESULTS:
The NO donor, sodium nitroprusside (SNP), reduced the [Ca2+]i in 72% of the cells tested (n=100). In the remaining cells, the effect of SNP was biphasic, or the [Ca2+]i was increased. In addition, the membrane-permeable cGMP, 8-bromo cGMP, decreased the [Ca2+]i. The effects of SNP and 8-bromo cGMP were inhibited by the soluble guanylate cyclase inhibitor, 1H-[1,2,4] oxadiazole[4,3-a]quinoxalin-1-one (ODQ), and the cGMP-dependent protein kinase inhibitor, KT5823, respectively. In contrast, in the presence of 8-bromo cGMP or ODQ, SNP increased the [Ca2+]i.CONCLUSION:
These results suggest that NO inhibits the [Ca2+]i through a cGMP-dependent mechanism and increases the [Ca2+]i through a cGMP-independent mechanism.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Quinases
/
Doadores de Tecidos
/
Nitroprussiato
/
GMP Cíclico
/
Miócitos de Músculo Liso
/
Células Endoteliais
/
Endotélio
/
Células Endoteliais da Veia Umbilical Humana
/
Guanilato Ciclase
/
Óxido Nítrico
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Korean Circulation Journal
Ano de publicação:
2004
Tipo de documento:
Artigo
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