Use of Human Skin Reconstructs Containing Melanocytes as a Model to Evaluate the Effect of Pigment Modifiers / 대한피부과학회지
Korean Journal of Dermatology
;
: 407-412, 2001.
Artigo
em Coreano
| WPRIM
| ID: wpr-129993
ABSTRACT
BACKGROUND:
Recently human skin reconstructs composed of keratinocytes and melanocytes have been developed.OBJECTIVE:
We investigated whether human skin reconstructs containing melanocytes could be useful to test the efficacy of drugs that alter pigmentation.METHODS:
Human skin reconstructs were made by culturing human keratinocytes and melanocytes on the top of de-epidermized dermis. Reagents that alter melanogenesis such as isobutyl methylxanthine, 1-oleoyl-2-acetyl-sn-glycerol, kojic acid were treated in the culture medium after 7 days of culture at the air-liquid interface. Macroscopic, histological, histochemical and immunohistochemical studies were performed.RESULTS:
The effect of pigment modifiers was demonstrated in human skin reconstructs as expected from in vivo data on skin color, numbers of melanocytes and melanin content.CONCLUSION:
These results indicate that this model is very useful to assess the efficacy of drugs that alter pigmentation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pele
/
Pigmentação
/
Queratinócitos
/
Derme
/
Indicadores e Reagentes
/
Melaninas
/
Melanócitos
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Dermatology
Ano de publicação:
2001
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS