Alum Directly Modulates Murine B Lymphocytes to Produce IgG1 Isotype
Immune Network
;
: 10-15, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-13003
ABSTRACT
Aluminum hydroxide (alum) is the most widely used adjuvant in human vaccines. Nevertheless, it is virtually unknown whether alum acts on B cells. In the present study, we explored the direct effect of alum on Ig expression by murine B cells in vitro. LPS-activated mouse spleen B cells were cultured with alum, and the level of isotype-specific Ig secretion, IgG1 secreting cell numbers, and Ig germ-line transcripts (GLT) were measured using ELISA, ELISPOT, and RT-PCR, respectively. Alum consistently enhanced total IgG1 production, numbers of IgG1 secreting cells, and GLTgamma1 expression. These results demonstrate that alum can directly cause IgG1 isotype switching leading to IgG1 production.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Baço
/
Imunoglobulina G
/
Ensaio de Imunoadsorção Enzimática
/
Vacinas
/
Linfócitos B
/
Contagem de Células
/
Switching de Imunoglobulina
/
Compostos de Alúmen
/
Hidróxido de Alumínio
/
ELISPOT
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Immune Network
Ano de publicação:
2013
Tipo de documento:
Artigo
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