Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors
Journal of Veterinary Science
;
: 59-65, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-13091
ABSTRACT
Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Virais
/
Testes de Neutralização
/
Vacinas Virais
/
Western Blotting
/
Imunização
/
Organismos Livres de Patógenos Específicos
/
Herpesvirus Humano 1
/
Herpesvirus Bovino 1
/
Imunidade Humoral
/
Vetores Genéticos
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2012
Tipo de documento:
Artigo
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