Role of Endogenous Histamine on the Pathogenesis in the Endotoxin-Induced Acute Lung Injury / 결핵
Tuberculosis and Respiratory Diseases
;
: 91-103, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-130914
ABSTRACT
BACKGROUND:
Histamine is widely distributed in the lung. It increases capillary permeability and the P-selectin expression on vascular endothelial cell surfaces. We studied the role of endogenous histamine on the pathogenesis of endotoxin-induced acute lung injury (ALI) in rats.METHODS:
We instilled either normal saline (control group) or lipopolysaccharide (3 mg/Kg, LPS group) to tracheas of Sprague-Dawley rats. H1-receptor blocker (mepyramine, 10 mg/Kg, H1RB group), H2-receptor blocker (ranitidine, 10 mg/Kg, H2RB group), and H3-receptor blocker (thioperamide, 2 mg/Kg, H3RB group) were administered through vein or peritoneum along with intratracheal LPS administration. Statistical significance was accepted at p<0.05.RESULTS:
LPS increases the histamine level in BAL fluid significantly at 2 h after the treatment compared with control group. LPS significantly increases protein concentration, PMN cell count in bronchoalveolar lavage (BAL) fluid, and myeloperoxidase (MPO) activity in the lung tissue at 6 h compared to control group. PMN cell count in BAL fluid and MPO activity in lung tissue were significantly lower in H2RB-group compared to LPS-group. However, protein concentration in BAL fluid showed no significant differences between the LPS alone and LPS with histamine receptor blockade.CONCLUSIONS:
Endogenous histamine might be involved in the recruitment of PMNs in LPS-induced ALI via H2 receptor. However, its role in ALI would not be significant in this model.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peritônio
/
Traqueia
/
Veias
/
Permeabilidade Capilar
/
Receptores Histamínicos
/
Histamina
/
Contagem de Células
/
Ratos Sprague-Dawley
/
Peroxidase
/
Selectina-P
Tipo de estudo:
Estudo de etiologia
Limite:
Animais
Idioma:
Coreano
Revista:
Tuberculosis and Respiratory Diseases
Ano de publicação:
2003
Tipo de documento:
Artigo
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