Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells
Journal of Veterinary Science
;
: 23-31, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-13096
ABSTRACT
Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclear cell (PBMC) proliferation was suppressed by hAM-MSCs in a dose-dependent manner as well as hAM-MSC culture supernatant. Moreover, interferon-gamma and interleukin (IL)-17 production significantly decreased from PBMC, whereas IL-10 from PBMCs and transforming growth factor beta (TGF-beta) production from hAM-MSCs significantly increased in co-cultures of hAM-MSCs and PBMCs. Production of several MSC factors, including hepatocyte growth factor (HGF), TGF-beta, prostaglandin E2 (PGE2), and indoleamine 2, 3 dioxygenase (IDO), increased significantly in hAM-MSCs co-cultured with PBMCs. These results indicate that the immunomodulatory effects of hAM-MSCs may be associated with soluble factors (TGF-beta, HGF, PGE2, and IDO), suggesting that hAM-MSCs may have potential clinical use in regenerative medicine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Leucócitos Mononucleares
/
Dinoprostona
/
Diferenciação Celular
/
Imunofenotipagem
/
Fator de Crescimento Transformador beta
/
Interferon gama
/
Interleucina-10
/
Fator de Crescimento de Hepatócito
/
Técnicas de Cocultura
Limite:
Feminino
/
Humanos
/
Gravidez
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2012
Tipo de documento:
Artigo
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