A Novel Compound Rasatiol Isolated from Raphanus sativus Has a Potential to Enhance Extracellular Matrix Synthesis in Dermal Fibroblasts
Annals of Dermatology
;
: 315-320, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-131877
ABSTRACT
BACKGROUND:
The fibrous proteins of extracellular matrix (ECM) produced by dermal fibroblast contributes to the maintenance of connective tissue integrity.OBJECTIVE:
This study is carried out to identify the bioactive ingredient from natural products that enhances ECM production in dermal fibroblasts.METHODS:
Bioassay-directed fractionation was used to isolate the active ingredient from natural extracts. The effects of rasatiol (isolated from Raphanus sativus) on ECM production in primary cultured human dermal fibroblasts was investigated by enzyme linked immunosorbent assay and western blot analysis.RESULTS:
Rasatiol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen, fibronectin and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was remarkably increased by rasatiol, indicating that enhanced ECM production is linked to the activation of intracellular signaling cascades.CONCLUSION:
These results indicate that rasatiol stimulates the fibrous components of ECM production, and may be applied to the maintenance of skin texture.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Fosfotransferases
/
Proteínas Quinases
/
Escleroproteínas
/
Pele
/
Ensaio de Imunoadsorção Enzimática
/
Western Blotting
/
Elastina
/
Fibronectinas
/
Tecido Conjuntivo
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Annals of Dermatology
Ano de publicação:
2013
Tipo de documento:
Artigo
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