Characterization of anti-factor VIII antibody in a patient with acquired hemophilia A
Blood Research
;
: 58-62, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-132566
ABSTRACT
Acquired hemophilia A (AHA) is a bleeding disorder caused by the development of an auto-antibody against endogenous factor VIII (FVIII). In this study, the epitope of the autoantibody was identified in a 67-year-old female patient with AHA. A prolonged activated partial thromboplastin time (77.4 s) that failed to correct in an incubation mixing test (68.2 s), a decreased FVIII activity, and a high FVIII inhibitor (14.6 Bethesda units/mL) were observed. Enzyme-linked immunosorbent assay demonstrated that the antibody belonged to the immunoglobulin G4 subclass. An immunoblotting assay revealed the light chain (A3/C1/C2 domain) of FVIII as the binding region of the antibody. The bleeding experienced by our patient resulted from the interference of FVIII binding to both FIX by anti-A3 antibodies and phospholipids and von Willebrand factor by anti-C2 antibodies. To the best of our knowledge, this is the first study in Korea characterizing an autoantibody in the context of AHA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tempo de Tromboplastina Parcial
/
Fosfolipídeos
/
Fator VIII
/
Imunoglobulinas
/
Fator de von Willebrand
/
Ensaio de Imunoadsorção Enzimática
/
Immunoblotting
/
Hemofilia A
/
Hemorragia
/
Coreia (Geográfico)
Tipo de estudo:
Estudo prognóstico
Limite:
Feminino
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Blood Research
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS