Detection of human herpesvirus 8 DNA in pemphigus and chronic blistering skin diseases
Journal of Korean Medical Science
;
: 442-448, 2000.
Artigo
em Inglês
| WPRIM
| ID: wpr-135349
ABSTRACT
Increased incidences of Kaposi's sarcoma and lymphoid malignancies have been observed in patients with pemphigus, and the human herpesvirus 8 (HHV-8) is very strongly associated with these tumors. Because the virus may be one of the triggering factors of pemphigus, we undertook this study to screen for the presence of HHV-8 in chronic blistering skin diseases including pemphigus. A total of 45 paraffin-embedded specimens were studied using nested polymerase chain reaction (PCR) with primers to amplify a 160-base pair HHV-8 fragment. HHV-8 DNA could be detected in 7 of 9 patients with pemphigus vulagris, and 1 of 2 with pemphigus foliaceus. All specimens of other blistering skin diseases were negative for HHV-8. On sequencing PCR products, the sequences were almost identical with the prototypic sequence for HHV-8, and a few base- pair substitutions at 1086C-T and 1139A-C were detected. The results of our study suggests that HHV-8 might have trophism for pemphigus lesions. Further studies including comparison of HHV-8 DNA load in both lesional and normal skin in the same patient, serological and animal studies would be helpful to study the relationship between HHV-8 and pemphigus.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA Viral
/
Estudo Comparativo
/
Análise Mutacional de DNA
/
Reação em Cadeia da Polimerase
/
Prevalência
/
Estudos Retrospectivos
/
Dermatopatias Vesiculobolhosas
/
Pênfigo
/
Fixação de Tecidos
/
Inclusão em Parafina
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
/
Estudo de prevalência
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Journal of Korean Medical Science
Ano de publicação:
2000
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS