Preparation and utilization of factor VIII rich cryoprecipitation

ABSTRACT

Plasma is separated fresh unit of whole blood in closed system. The plasma is frozen in system that will allow complete freezing within one hour to a temperature below-350C. The plasma is thawed slowly at 1-60 C for 18 h and centrifuged at 1-60 C. Final volume for cryoprecipitate concentrate is 10-20/ml. Result: 140 samples during 2 years 95.57% FVIII > 70IU/unit. 4.43% F VIII > 45 IU to 68 IU /unit. During 2 years 1999 to 2000 hospitals in HCM city have used 5,675 F VIII rich cryoprecipitate unit for treatment Hemophilia A, DIC, fibrinogen defects. Side effects as mainly chills, fever and urticaria have not reported. Results of treatment are very good and safe for patients.