Tumor associated proteins of rat skin tumor induced by 7,12-dimethylbenzaanthracene
Yonsei Medical Journal
;
: 219-234, 1989.
Artigo
em Inglês
| WPRIM
| ID: wpr-135966
ABSTRACT
The incidence of tumor and the time of expression, cellular localization and the molecular weight of tumor associated proteins of rat skin tumor induced by 7,12-dimethylbenz[a]anthracene (DMBA) with or without 12-O-tetradecanoyl-phorbol-13-acetate (TPA) were studied. The time of the development of skin tumors in 0.1% DMBA-TPA treated rats was significantly shorter than that in rats which were treated with DMBA alone. In the complete carcinogenesis case, papillomas developed more slowly and were less common and also squamous cell carcinomas appeared much later. From the analysis of the proteins of each experimental group by SDS-PAGE and two dimensional gel electrophoresis, at least three tumor associated proteins were identified (54kd, pl = 5.66; 27kd, pl = 5.85; 11kd, pl = 4.90). Also these proteins were found in rat dorsal skin from 14 days gestation to 21 days postpartum, and disappeared after 28 days. In conclusions, two stage skin carcinogenesis could be successfully demonstrated in Sprague-Dawley rats and abnormal proteins were produced in DMBA or DMBA-TPA induced skin tumor. The tumor associated proteins of skin tumor induced by DMBA or DMBA-TPA were appeared at the late initiation stage or early promotion stage, and they were localized in plasma membrane and were glycoproteins that are thought to be related to the epidermal differentiation process.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Papiloma
/
Ratos Endogâmicos
/
Neoplasias Cutâneas
/
Acetato de Tetradecanoilforbol
/
Estudo Comparativo
/
Glicoproteínas
/
Carcinoma de Células Escamosas
/
Membrana Celular
/
9,10-Dimetil-1,2-benzantraceno
/
Animais
Limite:
Animais
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
1989
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS