IFN-gamma Regulates Expression of BRG1 Associated Factor 155/170 and Sensitivity to Steroid in Astrocytes
Immune Network
;
: 224-228, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-13654
ABSTRACT
BACKGROUND:
The expression of BRG1 associated factors (BAF) 155 and BAF 170 in response to IFN-gamma or TNF-alpha was studied in astrocytoma cell lines and primary astrocytes. BAFs are complexed with BRG1 and are also associated with activated glucocorticoid for glucocorticoid trans-activation.METHODS:
IFN-gamma was pretreated for 18 hrs and cells were incubated with IL-1 or TNF-alpha for 72 hrs or 96 hrs with different concentrations of steroid. Cell death was measured by LDH assay. BAF expression was assayed by RT-PCR.RESULTS:
IFN-gamma increased cell death by dexamethasone in LN215 cells but not in LN319 cells. The IFN-gamma increased the expression of BAF 155 and BAF 170 in adult astrocytes and LN215 cells, but IFN-gamma decreased the expression of BAF 155/170 in LN319 cells. The effect of IFN-gamma on the expression of BAF was not as clear in fetal astrocytes as it was in adult astrocytes.CONCLUSION:
Our results suggest cytokines produced during immune reaction or immunotherapy may modulate steroid susceptibility of astrocytes and astrocytoma cells by influencing the expression of BAFs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Astrocitoma
/
Dexametasona
/
Linhagem Celular
/
Astrócitos
/
Citocinas
/
Interleucina-1
/
Fator de Necrose Tumoral alfa
/
Morte Celular
/
Imunoterapia
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Humanos
Idioma:
Inglês
Revista:
Immune Network
Ano de publicação:
2004
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS