Effect of Glutathione on Lead Induced Modulation of NO Synthesis in RAW 264.7 Cell / 예방의학회지
Korean Journal of Preventive Medicine
;
: 269-274, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-136621
ABSTRACT
OBJECTIVES:
To evaluate the effect of glutathione(GSH) on lead induced modulation of nitric oxide(NO) synthesis, and to examine how lead modulates NO production in macrophages.METHODS:
This study was observed in a culture of RAW 264.7 cells, which originated from a tumor in a Balb/c mouse that was induced by the Abelson murine leukemia virus. The compounds investigated were lead chloride, N-acetyl-cystein(NAC), and Buthionine Sulfoximine(BSO). RESUJLTS ATP synthesis in RAW 264.7 cells was unchanged by each lead concentration exposure in a dose dependent manner. The NO synthesis was decreased when exposed to lead(PbCl2) concentration 0.5 micro M. The presence of 300 micro M NAC, used as a pretreatment in the culture medium, caused the recovery of the lead induced decrease in NO synthesis, but in the presence of 300 micro M BSO as a pretreatment, there was no recoverey. Pretreatment with NAC and BSO had no affect on ATP synthesis at any of the lead concentrations used.CONCLUSIONS:
These results indicated that GSH has a protective effect toward lead toxicity, and suggested that the inhibition of NO production in macrophage due to lead toxicity may be related to cofactors of iNOS (inducible nitric oxide synthase)
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírus da Leucemia Murina de Abelson
/
Acetilcisteína
/
Trifosfato de Adenosina
/
Butionina Sulfoximina
/
Glutationa
/
Macrófagos
/
Óxido Nítrico
Limite:
Animais
Idioma:
Coreano
Revista:
Korean Journal of Preventive Medicine
Ano de publicação:
2002
Tipo de documento:
Artigo
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