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Quantitative and Qualitative Estimation of Bacteria Contaminating Human Hairs
Journal of Bacteriology and Virology ; : 11-18, 2010.
Artigo em Inglês | WPRIM | ID: wpr-136977
ABSTRACT
Human hairs have been known to be easily contaminated with microorganisms. This study was performed in order to measure what bacterial species and how much microorganisms contaminate human hairs in specific place. Virgin human hairs were left at 6 positions in inside corner and beside window in a laboratory for 7 days. The number of viable bacterial cells, which were determined by most probable number method, contaminating the human hairs was measured at a maximum of 10(6)/g hair and a minimum of 10(3)/g hair in inside corner and maximum of 10(6)/g hair and a minimum of 10(3)/g hair beside window. The bacterial cells-contaminating human hairs were observed via fluorescence light microscopy after 4',6-diamino-2-phenylindole (DAPI) staining. The bacterial community contaminating human hairs was analyzed via the thermal gradient gel electrophoresis (TGGE) technique, based on the diversity of the 16S-rDNA variable region. In total, approximately 20 bacterial species were detected from 12 groups of hair samples. In this study, general experimental methods-fluorescence staining, TGGE and MPN-were combined to develop new method for observation and estimation of bacteria contaminating human hairs.
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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Bactérias / Oftalmoplegia / Doenças Mitocondriais / Eletroforese / Fluorescência / Cabelo / Hipogonadismo / Luz / Microscopia Tipo de estudo: Pesquisa qualitativa Limite: Humanos Idioma: Inglês Revista: Journal of Bacteriology and Virology Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Bactérias / Oftalmoplegia / Doenças Mitocondriais / Eletroforese / Fluorescência / Cabelo / Hipogonadismo / Luz / Microscopia Tipo de estudo: Pesquisa qualitativa Limite: Humanos Idioma: Inglês Revista: Journal of Bacteriology and Virology Ano de publicação: 2010 Tipo de documento: Artigo