Inhibitory Effects of Siryungtang and Onbitang on Oxidation of Low Density Lipoprotein / 대한신장학회잡지

ABSTRACT

BACKGROUND: LDL deposition and local oxidation play a key role in the pathogenesis of atherosclerosis and glomerulosclerosis. Oxidation of LDL need to be catalyzed by copper or iron, whereas the antioxidants, such as probucol, superoxide dismutase, L-ascorbic acid, dehydro-L-ascorbic acid inhibit oxidation of LDL. Recently, the herbal medicines, such as Astragali Radix, Radix angelicae sinensis were shown to have inhibitory effects on lipid oxidation. So, we investigated the effects of Siryungtang and Onbitang on oxidation of LDL. METHODS: Siryungtang and Onbitang were selected. LDL was isolated from the plasma of normal volunteers by sequential ultracentrifugation by using potassium bromide(KBr) for density adjustment. The chemical oxidation of LDL was performed by the addition of 10micro-osmole CuSO4 in phosphate-buffered saline. The measurement of oxidized LDL used the thiobarbituric acid reactive substance assay(TBARS). TBARS were estimated on spectrophotometer at 532 nm and sample values were based on a liner regression of the standard curve(tetramethoxypropane). RESULTS: As the concentration of all of Siryungtang and Onbitang increased, TMP concentration inversely decreased. Siryungtang observed inhibitory effects on TMP from 50microgram/mL to 200microgram/mL and Onbitang from 100microgram/mL to 200microgram/mL. Antioxidant effect of Siryungtang was more excellent than that of Onbitang. CONCLUSION: Siryungtang and Onbitang inhibited oxidation of LDL. So, Siryungtang and Onbitang may attenuate the risk of atherosclerosis and glomerulosclerosis.