Effect of NG-nitro-L-arginine Methyl Ester Pretreatment on Spinal Cord Damage by Aortic Occlusion in Rabbits / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 1059-1066, 1999.
Artigo
em Coreano
| WPRIM
| ID: wpr-138208
ABSTRACT
BACKGROUND:
Production of nitric oxide (NO) radicals may contribute to neuronal injury. We examined that the inhibition of NO synthase (NOS) could improve postischemic neurologic outcome after spinal cord ischemia in rabbits. Also, we measured cGMP as a marker of NOS activation in control and experimental groups.METHODS:
Spinal cord ischemia in rabbits was induced by aortic occlusion with aneurysm clip at the level just below branching of left renal artery. Five minutes before aortic occlusion, saline (control group, n=10) or a NOS inhibitor NG-nitro-L-arginine methyl ester (10 mg/kg, L-NAME group, n=10) was injected intravenously. After 15 min ischemia and 1 hour reperfusion, animals were sacrified and the spinal cords were extruded for the measurement of cGMP by enzymeimmunoassay. For neurologic examination, the same procedures of ischemia/reperfusion and drug injection were done, except that rabbits were perfused for 4 hours (control-4 and L-NAME-4, n=8 at each group) or 48 hours (control-48 and L-NAME-48, n=8 at each group) after aortic occlusion.RESULTS:
L-NAME (10 mg/kg) increased mean systemic arterial pressure accompanied by bradycardia, and reduced cGMP significantly. Control-4 animals showed better neurologic function than L-NAME-4 animals (p<0.05), however, there was no significant difference of neurologic outcome between control-48 and L-NAME-48 groups.CONCLUSION:
Intravenous administration of L-NAME prior to spinal cord ischemia/reperfusion diminishes the extent of postischemic neuronal spinal cord damage at the early postreperfusion period.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Artéria Renal
/
Medula Espinal
/
Bradicardia
/
Reperfusão
/
Óxido Nítrico Sintase
/
NG-Nitroarginina Metil Éster
/
Nitroarginina
/
Isquemia do Cordão Espinal
/
Administração Intravenosa
/
Pressão Arterial
Limite:
Animais
Idioma:
Coreano
Revista:
Korean Journal of Anesthesiology
Ano de publicação:
1999
Tipo de documento:
Artigo
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