Compound K attenuates stromal cell-derived growth factor 1 (SDF-1)-induced migration of C6 glioma cells
Nutrition Research and Practice
; : 259-264, 2016.
Article
em En
| WPRIM
| ID: wpr-138396
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WPRO
ABSTRACT
BACKGROUND/OBJECTIVES: Stromal cell-derived growth factor 1 (SDF-1), also known as chemokine ligand 12, and chemokine receptor type 4 are involved in cancer cell migration. Compound K (CK), a metabolite of protopanaxadiol-type ginsenoside by gut microbiota, is reported to have therapeutic potential in cancer therapy. However, the inhibitory effect of CK on SDF-1 pathway-induced migration of glioma has not yet been established. MATERIALS/METHODS: Cytotoxicity of CK in C6 glioma cells was determined using an EZ-Cytox cell viability assay kit. Cell migration was tested using the wound healing and Boyden chamber assay. Phosphorylation levels of protein kinase C (PKC)α and extracellular signal-regulated kinase (ERK) were measured by western blot assay, and matrix metallopeptidases (MMP) were measured by gelatin-zymography analysis. RESULTS: CK significantly reduced the phosphorylation of PKCα and ERK1/2, expression of MMP9 and MMP2, and inhibited the migration of C6 glioma cells under SDF-1-stimulated conditions. CONCLUSIONS: CK is a cell migration inhibitor that inhibits C6 glioma cell migration by regulating its downstream signaling molecules including PKCα, ERK1/2, and MMPs.
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Assunto principal:
Fosforilação
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Fosfotransferases
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Cicatrização
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Proteína Quinase C
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Movimento Celular
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Sobrevivência Celular
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Western Blotting
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Metaloproteinases da Matriz
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Metaloproteases
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Microbioma Gastrointestinal
Idioma:
En
Revista:
Nutrition Research and Practice
Ano de publicação:
2016
Tipo de documento:
Article