Targeted Plasma Metabolite Profiling of Metformin in Healthy Korean Volunteers
Journal of Korean Society for Clinical Pharmacology and Therapeutics
;
: 175-181, 2012.
Artigo
em Coreano
| WPRIM
| ID: wpr-138499
ABSTRACT
BACKGROUND:
Metformin is an effective oral antihyperglycaemic agent for type 2 diabetes mellitus, with a variety of metabolic effects. In addition to controlling blood glucose level, it has been appeared to decrease the long-period complications of diabetes, including macrovascular disease. Few reports have addressed the metabolite profiling of metformin. The study was to evaluate if targeted metabolic profiling approach is sensitive enough to predict the therapeutic effects of metformin after a single oral dose.METHODS:
A randomized, open-label, single-dose study was conducted in twenty eight healthy Korean male volunteers. To determine the concentrations of endogenous metabolites in their pre-dose and post-dose plasma samples, blood samples were collected before and at 2 and 6 h after a single oral dose of 500 mg metformin. Both Modular P/Modular D analyzer and ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)-based metabolic profiling was performed.RESULTS:
We quantified pre-dose and post-dose creatinine, blood urea nitrogen (BUN), lactic acid, 7 amino acids (lysine, glutamic acid, alanine, valine, leucine, phenylalanine, tryptophan), and 5 lysophosphatidylcholines (140, 160, 170, 180, and 181) using autoanalyser and UPLC-MS/MS. The postdose levels of alanine, lactic acid, glutamic acid, lysine, valine, leucine, phenylalanine, tryptophan, and lysoPC (181) were slightly decreased with statistical significance, but there is no clinical significance.CONCLUSION:
In order to explore the potential endogenous metabolites associated with the therapeutic effects of metformin, further study including non-targeted (global) metabolite profiling is needed.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenilalanina
/
Plasma
/
Triptofano
/
Valina
/
Glicemia
/
Nitrogênio da Ureia Sanguínea
/
Lisofosfatidilcolinas
/
Cromatografia Líquida
/
Ácido Glutâmico
/
Ácido Láctico
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Journal of Korean Society for Clinical Pharmacology and Therapeutics
Ano de publicação:
2012
Tipo de documento:
Artigo
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