A decrease in the expression of CD63 tetraspanin protein elevates invasive potential of human melanoma cells
Experimental & Molecular Medicine
;
: 317-323, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-13850
ABSTRACT
CD63, which belongs to the tetraspanin membrane proteins, has been proposed to play an important role in inhibiting melanoma metastasis. To determine whether reduction of CD63 expression, which frequently occurs in the malignant progression of human melanoma, is responsible for metastasis promotion, we transfected the antisense CD63 cDNA into MelJuso melanoma cells having endogenous CD63 expression. The antisense CD63 transfectant clones showing decreased CD63 expression displayed increased cell motility, matrix-degrading activity, and invasiveness in vitro when compared with the control transfectant cells. The antisense CD63 cDNA-transfected cells also exhibited altered adhesiveness to extracellular matrix. The results suggest that reduced CD63 expression contributes to the invasive and metastatic ability of human melanoma cells.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Glicoproteínas da Membrana de Plaquetas
/
Antígenos CD
/
Regulação Neoplásica da Expressão Gênica
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Melanoma
/
Invasividade Neoplásica
Limite:
Humanos
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2003
Tipo de documento:
Artigo
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