Increased glycosylation of human lens epithelial basement membrane in diabetes mellitus
Korean Journal of Ophthalmology
; : 26-31, 1994.
Article
em En
| WPRIM
| ID: wpr-139565
Biblioteca responsável:
WPRO
ABSTRACT
We studied the nonenzymatic glycosylation of lens epithelial basement membranes (LEBM) of senile cataractous lenses of both diabetic and nondiabetic patients. The human LEBMs were isolated from surgically removed senile cataracts and purified by osmotic lysis and detergent treatments. Glycosylation assay of LEBMs was done using the colorimetric method of Fluckiger and Winterhalter. The glycosylation value ranged from 16.39 to 92.56 n mol/mg protein overall, with a mean of 63.54 +/- 24.56 n mol/mg protein for the diabetic specimens and a mean of 29.97 +/- 14.48 n mol/mg protein for the nondiabetic controls (P = 0.009). The study confirms our previous observation of in vivo glycosylation of the LEBM and further establishes that diabetic patients have a twofold increase in the amount of LEBM glycosylation when compared to their nondiabetic counterparts.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Membrana Basal
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Catarata
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Glicosilação
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Diabetes Mellitus Tipo 2
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Epitélio
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Glucose
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Cristalino
Limite:
Aged
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Female
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Humans
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Male
Idioma:
En
Revista:
Korean Journal of Ophthalmology
Ano de publicação:
1994
Tipo de documento:
Article