Effects of chronic alcohol and excessive iron intake on mitochondrial DNA damage in the rat liver / 한국영양학회지
Journal of Nutrition and Health
;
: 390-397, 2015.
Artigo
em Coreano
| WPRIM
| ID: wpr-14031
ABSTRACT
PURPOSE:
In this study, we investigated the effects of chronic alcohol and excessive iron intake on mitochondrial DNA (mtDNA) damage and the progression of alcoholic liver injury in rats.METHODS:
Twenty-four Sprague-Dawley male rats were divided into four groups (Control, EtOH, Fe, and EtOH + Fe), and fed either control or ethanol (36% of total calories) liquid diet with or without 0.6% carbonyl iron for eight weeks. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, liver malondialdehyde concentrations were measured by colorimetric assays. Liver histopathology was examined by Hematoxylin-eosin staining of the fixed liver tissues. The integrity of the hepatic mtDNA and nuclear DNA was measured by long-range PCR. The gene expression levels of cytochrome c oxidase subunit 1 (Cox1) and NADH dehydrogenase subunit 4 (Nd4) were examined by real-time PCR.RESULTS:
Serum ALT and AST activities were significantly higher in the EtOH+Fe group, as compared to the Control group. Similarly, among four groups, liver histology showed the most severe lipid accumulation, inflammation, and necrosis in the EtOH + Fe group. PCR amplification of near-full-length (15.9 kb) mtDNA showed more than 50% loss of full-length product in the liver of the EtOH + Fe group, whereas amounts of PCR products of a nuclear DNA were unaffected. In addition, the changes in the mtDNA integrity showed correlation with reductions in the mRNA levels of mitochondrial gene Cox1 and Nd4.CONCLUSION:
Our data suggested that the liver injury associated with excessive iron and alcohol intake involved mtDNA damage and corresponding mitochondrial dysfunction.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aspartato Aminotransferases
/
DNA
/
DNA Mitocondrial
/
RNA Mensageiro
/
Expressão Gênica
/
Reação em Cadeia da Polimerase
/
Ratos Sprague-Dawley
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Dieta
/
Alanina Transaminase
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Journal of Nutrition and Health
Ano de publicação:
2015
Tipo de documento:
Artigo
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