The Factors Affecting Transplanted Hepatocytes Repopulation in Rats with Liver Fibrosis
Korean Journal of Hepato-Biliary-Pancreatic Surgery
;
: 189-197, 2009.
Artigo
em Coreano
| WPRIM
| ID: wpr-140610
ABSTRACT
PURPOSE:
Cell therapy for various diseases has gained wide acceptance. Because most patients with chronic liver failure have mild-to-severe liver cirrhosis, there are many limitations to clinical applications. We analyzed how to increase cell engraftment in rats with liver fibrosis.METHODS:
We used analbuminemic SD rats (NARs) as recipients of syngeneic CAG-EGFP SD hepatocytes obtained by the 2 perfusion method. Hepatic fibrosis was induced with thioacetamide in drinking water for 6 weeks in the recipient NARs. NARs were pre-treated with gadolinium, doxorubicin, and gliotoxin before hepatocyte transplantation. We evaluated the degree of cell engraftment by RT-PCR and immunofluorescent staining for GFP and albumin. The transplanted cells were detected by immunostaining for albumin, and serum albumin was also measured.RESULTS:
Although detection of GFP by RT-PCR was variable, albumin was detected in all groups 4 wks after hepatocyte transplantation. GFP and albumin were also detected by immunofluorescent staining 1 and 4 wks after cell transplantation. In control rats, albumin production was maximal at 3 wks, and after that it rapidly decreased. In the gadolinium and doxorubicin-treated group, albumin production was increased up to 4 wks. Albumin production in the gadolinium-treated group was superior to that of the doxorubicin-treated group.CONCLUSION:
Kupffer cells play the most important role in cell engraftment in hepatic fibrosis. Therefore, perturbation of kupffer cells in hepatic fibrosis is needed to increase cell engraftment.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Perfusão
/
Tioacetamida
/
Água Potável
/
Fibrose
/
Albumina Sérica
/
Doxorrubicina
/
Transplante de Células
/
Transplantes
/
Hepatócitos
/
Doença Hepática Terminal
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Hepato-Biliary-Pancreatic Surgery
Ano de publicação:
2009
Tipo de documento:
Artigo
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