Phylogenetic Analysis of env Gene V3-V5 Region of HIV-1 Subtype A Isolates from Korean
Journal of the Korean Society of Virology
;
: 119-127, 1999.
Artigo
em Coreano
| WPRIM
| ID: wpr-142034
ABSTRACT
Phylogenetic analysis was conducted to monitor transmission of HIV and to investigate the genetic structure of primary isolates from 12 HIV-1 subtype A infected Koreans. The individuals infected with subtype A viruses had been diagnosed as HIV-1 seropositives during the period 1987 to 1995 and blood samples have been collected from 1991 to 1997. DNA of each individual was isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 rev gene was amplified by nested polymerase chain reaction and the PCR products were sequenced. The mean value of the divergence of nucleotide of HIV-1 euv V3-V5 fragment was 17.0+/-4.06% (8.6~25.8%) within HIV-1 subtype A isolates from Koreans. This diversity was higher than those of African isolates (13.7+/-2.66%). In the phylogenetic tree, Korean subtype A isolates were not grouped together, but intermingled into African isolates. The results of this study suggested that HIV-1 subtype A variants be introduced from multiple sites of Africa into Korea and the big genetic diversity of Korea HIV-1 subtype A isolates may be further influenced by the range of geographic locations in which the infection occurred rather than the elapsed time between infection and collection of samples and the disease progression.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Variação Genética
/
DNA
/
Genes env
/
Genes rev
/
Reação em Cadeia da Polimerase
/
HIV-1
/
HIV
/
Progressão da Doença
/
África
/
Estruturas Genéticas
País/Região como assunto:
África
/
Ásia
Idioma:
Coreano
Revista:
Journal of the Korean Society of Virology
Ano de publicação:
1999
Tipo de documento:
Artigo
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