Evaluation of antibacterial activity and osteoblast-like cell viability of TiN, ZrN and (Ti(1-x)Zr(x))N coating on titanium
The Journal of Advanced Prosthodontics
;
: 166-171, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-144359
ABSTRACT
PURPOSE:
The aim of this study was to evaluate antibacterial activity and osteoblast-like cell viability according to the ratio of titanium nitride and zirconium nitride coating on commercially pure titanium using an arc ion plating system. MATERIALS ANDMETHODS:
Polished titanium surfaces were used as controls. Surface topography was observed by scanning electron microscopy, and surface roughness was measured using a two-dimensional contact stylus profilometer. Antibacterial activity was evaluated against Streptococcus mutans and Porphyromonas gingivalis with the colony-forming unit assay. Cell compatibility, mRNA expression, and morphology related to human osteoblast-like cells (MG-63) on the coated specimens were determined by the XTT assay and reverse transcriptase-polymerase chain reaction.RESULTS:
The number of S. mutans colonies on the TiN, ZrN and (Ti(1-x)Zr(x))N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05).CONCLUSION:
The number of P. gingivalis colonies on all surfaces showed no significant differences. TiN, ZrN and (Ti(1-x)Zr(x))N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
Streptococcus mutans
/
Estanho
/
Titânio
/
Zircônio
/
RNA Mensageiro
/
Microscopia Eletrônica de Varredura
/
Sobrevivência Celular
/
Porphyromonas gingivalis
/
Biofilmes
Limite:
Humanos
Idioma:
Inglês
Revista:
The Journal of Advanced Prosthodontics
Ano de publicação:
2015
Tipo de documento:
Artigo
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