An improved multiplex PCR for diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis / 대한수의학회지
Korean Journal of Veterinary Research
;
: 39-43, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-145338
ABSTRACT
A multiplex PCR was developed for the simultaneous detection and differentiation of Mycoplasma (M.) hyopneumoniae and M. hyorhinis in clinical samples. Improved sensitivity is advantage of this technique over the previously reported multiplex assay. It was capable of detecting as little as 125 fg genomic DNA from M. hyopneumoniae and 62.5 fg genomic DNA from M. hyorhinis. Application of this multiplex PCR method to field isolates showed that M. hyopneumoniae and M. hyorhinis were present in 29% (107 of 370) of lung specimens and no mycoplasmas were detected in 56% (208 of 370) of the slaughtered pigs' lungs. At the farm level, M. hyopneumoniae and M. hyorhinis were detected in 34 of 36 (94.4%) randomly selected farms. We conclude that this assay would prove itself a value tool for monitoring these mycoplasmal infections and both M. hyopneumoniae and M. hyorhinis have been widely spread in swine herds of Korea.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
DNA
/
Mycoplasma hyopneumoniae
/
Mycoplasma hyorhinis
/
Reação em Cadeia da Polimerase Multiplex
/
Imidazóis
/
Coreia (Geográfico)
/
Pulmão
/
Mycoplasma
/
Nitrocompostos
Tipo de estudo:
Estudo diagnóstico
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Korean Journal of Veterinary Research
Ano de publicação:
2012
Tipo de documento:
Artigo
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