Diagnosis of canine brucellosis using recombinant ribosomal protein L7/L12 / 대한수의학회지
Korean Journal of Veterinary Research
;
: 25-31, 2012.
Artigo
em Coreano
| WPRIM
| ID: wpr-145340
ABSTRACT
Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Placenta
/
Proteínas Ribossômicas
/
Brucella
/
Brucelose
/
Ensaio de Imunoadsorção Enzimática
/
Sensibilidade e Especificidade
/
Células Clonais
/
Médicos Veterinários
/
Feto Abortado
/
Brucella canis
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Veterinary Research
Ano de publicação:
2012
Tipo de documento:
Artigo
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