Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice
Experimental & Molecular Medicine
;
: 588-592, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-145919
ABSTRACT
Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S116I, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S116I transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Camundongos Transgênicos
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Expressão Gênica
/
Proteínas do Core Viral
/
Sequência de Aminoácidos
/
Hepatite C
/
Transgenes
/
Hepatócitos
Limite:
Animais
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2004
Tipo de documento:
Artigo
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