Oxidative stress effect on the activation of hepatic stellate cells
Yonsei Medical Journal
;
: 1-8, 2001.
Artigo
em Inglês
| WPRIM
| ID: wpr-147215
ABSTRACT
Collagen is the most excessive extracellular matrix protein in hepatic fibrosis. Activated, but not quiescent, hepatic stellate cells (HSCs) have a high level of collagen and a smooth muscle actin (alpha SMA) expression. HSCs play a key role in the pathogenesis of hepatic fibrosis. We analyzed a mechanism leading to HSC activation by evaluating the role of oxidative stress and the expression of NFkB. In vitro study HSCs were proliferated (PCNA2% vs 68%) and activated (alpha SMA 5% vs 78%) by ascorbate/FeSO4, and HSCs activated by type I collagen were blocked (PCNA 97% vs 4%, a SMA 86% vs 9%) by a-tocopherol. In vivo study means of a SMA positive cells in liver at 400 x HPF were 48.3+/-5.2 and 15.2+/-1.8 and [3H]thymidine uptake of HSC was 529.2+/-284.8 cpm and 223.0+/-86.3 cpm in control and a-tocopherol treated group respectively at 32 hours after CCl4 injection. Nuclear extracts from activated, but not from quiescent, HSCs formed a complex with the NFkB cognate oligonucleotidesand alpha-tocopherol inhibited this bindings. This study indicates that oxidative stress plays an essential role through the induction of NFkB on HSC activation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vitamina E
/
Células Cultivadas
/
Proteínas da Matriz Extracelular
/
NF-kappa B
/
Actinas
/
Ratos Sprague-Dawley
/
Estresse Oxidativo
/
Fígado
/
Cirrose Hepática
/
Animais
Limite:
Animais
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2001
Tipo de documento:
Artigo
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