A Case of Emetic Toxin Producing Bacillus cereusStrains Isolated from Outbreak / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
;
: 48-52, 2009.
Artigo
em Coreano
| WPRIM
| ID: wpr-147504
ABSTRACT
Bacillus cereus causes two types of gastrointestinal diseases emesis and diarrhea. It produces one emetic toxin and nine different enterotoxins. In March 2008, eight of a family became sick after eating slices of raw fish. We isolated emetic toxin producing B. cereus from the stools of 6 patients and 2 subclincal humans. In this study, the presence of enterotoxin genes, such as those of haemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe), B. cereus enterotoxin T (BceT), enterotoxin FM (EntFM), cytotoxin K (cytK) and cereulide were assayed by polymerase chain reaction (PCR) methods. Their enterotoxin activities were assayed using the BCET- RPLA, Tecra ELISA kit and Hep-2 vacuole activity. Bacterial isolates were subtyped by pulsed-field gel electrophoresis (PFGE). This study demonstrates the emetic toxin-producing stains of B. cereus in clinical specimens, for the first time in the Republic of Korea.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bacillus
/
Bacillus cereus
/
Vacúolos
/
Vômito
/
Ensaio de Imunoadsorção Enzimática
/
Reação em Cadeia da Polimerase
/
Eletroforese em Gel de Campo Pulsado
/
Depsipeptídeos
/
Diarreia
/
Corantes
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Coreano
Revista:
Korean Journal of Clinical Microbiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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