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Tacrolimus (FK506) Induced Apoptotic Signal Transduction Pathway
Journal of the Korean Surgical Society ; : 359-365, 2007.
Artigo em Coreano | WPRIM | ID: wpr-148074
ABSTRACT

PURPOSE:

This study examined the effects of Tacrolimus (FK506) on the expression of the apoptotic signal transduction proteins of Jurkat human T-lymphocytes.

METHODS:

The cell viability was examined by a MTT assay, DAPI stain, enzyme activity of caspase family proteins, and western blotting for Bcl-2, Bak, Fas, and Fas-L. The cells were cultured in the presence or absence of FK506. FK506 induced cell death was confirmed to be apoptosis by the observation of nuclear fragmentation.

RESULTS:

The viability of Jurkat cells was decreased by the addition of FK506 in a dose- and time- dependent manner. The FK506 induced activation of caspase-3 protease was observed. FK506 didn't increase the catalytic activity of caspase -6, -8, and -9 proteases of Jurkat cells in a time-dependent manner. The viability was improved when a caspase-3 inhibitor was added. However, the caspase-9 inhibitor did not affect the viability. Bak protein expression was increased, and the Bcl-2 protein was decreased for some time. The expression of Fas and Fas-L were unaffected by FK506.

CONCLUSION:

FK506 induces dose- and time-dependent apoptotic cell death, and enhances the apoptosis of Jurkat cell by increasing the expression of Bak and caspase-3.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peptídeo Hidrolases / Linfócitos T / Transdução de Sinais / Sobrevivência Celular / Western Blotting / Tacrolimo / Morte Celular / Apoptose / Células Jurkat / Proteína Killer-Antagonista Homóloga a bcl-2 Limite: Humanos Idioma: Coreano Revista: Journal of the Korean Surgical Society Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peptídeo Hidrolases / Linfócitos T / Transdução de Sinais / Sobrevivência Celular / Western Blotting / Tacrolimo / Morte Celular / Apoptose / Células Jurkat / Proteína Killer-Antagonista Homóloga a bcl-2 Limite: Humanos Idioma: Coreano Revista: Journal of the Korean Surgical Society Ano de publicação: 2007 Tipo de documento: Artigo